Technique of Calibrating Pipette

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Published: 03rd May 2011
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Calibration is a generally misinterpreted part of the maintenance routine of pipettes. It's because pipettes are calibrated depending on a particular standard and even as per strict polices. The genuine procedure of pipetting in a laboratory work (after the pipette calibration and service) might cause problem and even indecisiveness by producing different outcomes. As an example, a pipette calibrated by experienced personnel in a humidity and even temperature-controlled atmosphere based on the manufacturer's guidance is highly more likely to produce distinct outcomes if it is taken out of the freezer and even used in a warm lab.

This, however, is definitely an extreme and possibly unlikely instance, however, some of its aspects can be seen even in normal labs. As well as since there are no normal laboratories, you must inform the calibration company related to any specific needs you've got for pipette calibration. These specifications might include about the sorts of liquids to be utilized or any other important guidelines or else approved standards to be used by the calibration company.

Ways of pipette calibration

Calibration is often executed in ISO17025-accredited labs by gravimetric analysis, a typically used technique. For non-accredited calibration, colorimetric methods are used for checking pipettes. Many laboratories opt to work with gravimetric analysis because of it's the traceability to an absolute standard and even comparative simplicity. Gravimetric analysis is even known to be alot more cost-effective than others.

This system involves putting distilled water in the receiving vessel in a accurate analytical balance. The known constant is the density of water, while the moisture, heat and barometric pressure are recorded and even managed within certain limits. The mass of the sterilized water samples gives a detailed idea of the volume which has been dispensed.

All the participatory components in the procedure of pipette calibration are audited for precise measurements as per compliance and even chain of custody standards. In order to ensure the complete reliability of all operation levels, external bodies audit the thermometers, micro-balances, barometric devices, information recorders, users and even software. The results of pipette calibration turn out to be even more trusted if your procedure is conducted under controlled conditions. Calibration done in special 'Calibration Rooms' that have vibration-free surfaces and even the right environment delivers superior and even more effective outcome.

In-house pipette calibration

Lots of organizations manage their pipette servicing and even calibration on their own. In-house calibration, however, have to be done provided that you have the right equipment and even professional employees to carry out the calibration in recorded and even supervised conditions. Balances using the best suited software and even 'evaporation traps' can be purchased for this purpose. Ideally, this software should contain monitoring/training options as well as products control so your employees can carry on with the good methods.

Pipette calibration tips

  1. The entire process of calibration is highly affected by the viscosity and even temperature of the analyze liquid.


  2. Pipette producers do not possess steady standards on which to base their functionality claims.


  3. The more skilful the pipette user, the more valid the results.


  4. The entire process of calibration is also affected by the type of pipette you choose. It is usually advisable to make use of the tip provided by the manufacturer.

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